Probiotics, Nutrition and Microbes

Biography

Biography: Gabriela Krausova

Abstract

Poster Presentation:

Bifidobacteria and lactobacilli isolates were obtained from samples of cowËŠs colostrum, excrements and saliva of calves and piglets, as well as from infant´s faeces. Individual strains were identified using MALDI-TOF and characterized for their properties having an affinity to adhesion. The most frequently isolated strain was Lactobacillus reuteri and Lactobacillus casei subsp. paracasei. Bifidobacteria were isolated only from calves and piglets, B. thermophilum and B. pseudolongum from their excrements and B. longum from saliva of calves. To determine adhesion properties of selected strains in vitro, the mixture of Caco-2 and HT-29-MTX cell culture was used as a model. Furthermore, the method of microbial adhesion to hydrocarbons (MATH) - hydrophobicity and autoaggregation properties of the strains were tested. Generally, all tested isolates (n=20) were able to adhere to the tissue model in vitro. However, a great diversity in adherence ability among individual strains was observed. The highest adherence capacity (37.22%) was found in the Lactobacillus amylovorus strain originating from cow´s colostrum. Adherence above 30% was observed primarily in strains islated from infant´s faeces and subsequently, from colostrum. The highest hydrophobicity (˃ 90%) was determined for the B. thermophilum T11B strain (excrements of calves) and high hydrophobicity (˃ 70%) was also found in the K18 L. reuteri (colostrum) and S4B B. thermophilum (excrements of piglet) strains. The highest percentage of autoaggregation was observed in strains of lactobacilli isolated from colostrum. Perspective strains will be further characterized focusing on their properties important for potential probiotic strains.