Yazdi M H
Pasteur Institute of Iran
Iran
Title: Lactobacillus acidophilus stimulated Th1 cytokine production in BALB/c mice bearing breast cancer
Biography
Biography: Yazdi M H
Abstract
Background & Aims: The immunomodulation effect of Lactobacillus acidophillus as a probiotic was under evaluation in present work. We have studied the effect of oral administration of L. acidophilus on the immune responses and pattern of cytokine production in BALB/c mices bearing breast cancer.
Methods: 6-8-week-old in-bred BALB/c mice, each weighing 25-30 g were obtained from Pasture Institute Iran .They were divided in 2 groups and each experimental group consisted of 9 mice while 9 mice were used as controls in each assay. The L. acidophilus ATCC4356 strain has been used in this study. L. acidophilus was inoculated in MRS broth and cultivated for a day at 37° C under anaerobic conditions, collected by centrifugation and re-suspended in PBS. After preparation of proper amount of this suspension it was orally administered to the, control mice received an equal volume of PBS in the same manner.
Results: Result of this study showed that oral administration of Lactobacillus acidophilus as a potent immune-stimulator agent can motivated the proliferation of immune cells and increased the production of IFnγ instead of decrease in IL4 and TGFβ concentration which known as Th2 cytokine in spleen cell culture in addition as a most important point survival time of L. acidophilus treated mice have increased in comparison to control.
Conclusion: Our result suggested that daily use of Lactobacillus acidophilus can promoted immune responses with Th1 bias and may be increased the anti-tumour response and consumption of this probiotic strain maybe helpful for breast cancer patient but further studies is needed to investigate the other mechanisms of this effect.