K B M Saiful Islam
Sher-e-Bangla Agricultural University
Bangladesh
Title: Isolation and screening of potential probiotic bacteria from Bangladeshi indigenous poultry and subsequent in vitro studies
Biography
Biography: K B M Saiful Islam
Abstract
The use of imported probiotics in poultry is gradually being increased in Bangladesh. But surprisingly, despite the existence of scavenging native poultry that are apparently more resistant to GIT infection and other diseases as potential source, no probiotic has been developed yet in Bangladesh. Therefore, the study was undertaken to isolate, identify and characterize potential probiotic bacteria from Bangladeshi indigenous poultry and to evaluate their suitability to use in local poultry industry. A total 300 isolates belonging to the genera Lactobacillus and Bifidobacteria were identified following physical, biochemical and molecular methods from the crop and cecal samples of 61 healthy indigenous birds. Based on the auto-aggregation test, 12 lactobacilli and 7 bifidobacteria isolates were selected for antibacterial activity, resistance to low pH, resistance to bile extract, ability to produce H2O2, cell surface hydrophobicity and adhesion activities on crop epithelial cells to evaluate their probiotic potentials. Under the in vitro conditions and with respects to the probiotic traits, Lactobacillus salivarius, L. crispatus, L. johnsonii, Bifidobacterium thermophilum and B. bifidum were found to be potential probiotic strains. The probiotic isolates were found to tolerate stresses like acid, bile salt, lysozyme and NaCl thus indicating their persistence under in vivo conditions. The study findings seem to be highly motivating since they unveil the potentials of Bangladeshi isolates as probiotics. If the proposed strains could be used as probiotics in poultry, it is expected that the local probiotics would be more beneficial and would save the huge foreign currency that Bangladesh spends every year for the importation of such materials from abroad.