Day 1 :
Universidad Autonoma de Baja California Sur, Mexico
Keynote: Predominant lactic acid bacteria with probiotic potential for children, animal production and aquaculture in an arid coast region
Time : 09:20-10:00
Maurilia Rojas Contreras is a PhD in microbiology from the University of Gothenburg, Sweden. She is a research professor at Universidad Autonoma de Baja California Sur, Mexico since 1997. She has published in international journals regardless lactic acid bacteria, Lactobacillus adhesion to mucus, and probiotics for aquaculture. The main scientific question to answer is to understand the mechanism by which probiotic bacteria benefits your host.Maurilia Rojas Contreras is a PhD in microbiology from the University of Gothenburg, Sweden. She is a research professor at Universidad Autonoma de Baja California Sur, Mexico since 1997. She has published in international journals regardless lactic acid bacteria, Lactobacillus adhesion to mucus, and probiotics for aquaculture. The main scientific question to answer is to understand the mechanism by which probiotic bacteria benefits your host.
Statement of the Problem: Arid and semi-arid regions represent one third of the total area of the world including Australia, the southwest of the United States and northwest of Mexico. Probiotics able to survive in these conditions are important to maintain the health of humans and animals inhabiting the isolated arid coasts. Lactic acid bacteria (LAB) include strains from different genera that colonize mammals, birds, and marine animals and are broadly used as probiotic. The aim of this research was to perform a screening and selection of LAB with probiotic potential from faces and intestinal mucus of humans and organisms important for aquaculture and animal production in the northwest of Mexico, an arid coast. Methodology & Theoretical Orientation: Fecal and gastrointestinal mucus samples were collected of at least 10 subjects of humans, piglets, goats, calves, fish, shrimps, oysters and sea cucumber. Viable counts of LAB were performed and predominant bacteria were isolated and assayed for their ability to adhere gastrointestinal mucus from the respective host. Adhering bacteria were genetically identified by 16S rDNA sequence analysis. Findings: Results of viable counts showed an average of 7.0 and 4.0 log CFU/g of LAB in land and marine animals respectively. Isolates of predominant LAB in all species resulted in 1,031strains and 59% of them presented the ability to bind gastrointestinal mucus. LAB with high ability to bind mucus and more frequently isolated were Lactobacillus fermentum, L. Plantarum, Enterococcus faecium, L. Reuteri and L. Salivarius. Conclusion & Significance: These results indicated a rich source of potential probiotics that resist adverse environmental conditions and colonize the intestinal tract of organisms inhabiting isolated arid coasts.
Kaposvar University, Hungary
Keynote: Comparative studies on diverse Propolis samples in order to reveal their antimicrobial feature by means of a novel in vitro human intestinal model
Time : 10:00-10:40
Attila Kiss is a Professor of food chemistry. He is the Director of Institute Food Science Innovation Centre at Kaposvár University, Hungary. He obtained various experiences as the leader of the Food Science Research Institute of the National Agricultural Research and Innovation Centre’s Institute for Food Sciences, as well as the EGERFOOD Regional Knowledge Centre at Eszterházy Károly College. The scientific topics of his interest are development of functional foodstuffs of health-promoting effects, assessment of bioactive substances, food chemistry, transformation processes of food bioactive components and food safety issues. His impact factor is 71,7. Delivers regularly talks on Hungarian and international conferences (272 times).
Propolis is a natural product deriving from plant resins collected by honeybees. Propolis has been applied in the traditional medicine since ancient times, and at present gains growing popularity in healthy foods owing to its beneficial composition and plausible antimicrobial character. In this paper the antimicrobial properties of four different Hungarian propolis samples and their extracts were examined. We investigated the effects of various Hungarian propolis samples on a model microbiota of the large intestine. Until recently, only very few data was published about the impact of propolis on intestinal bacteria.
Agar diffusion test was applied to assess the inhibition zones in order to evaluate the impact of propolis samples on various bacteria strains. Influence of digestion on the antimicrobial activity was assessed by means of an improved in vitro model system simulating the digestion process by a three-step procedure.
Most of the investigated propolis samples exhibited inhibitory activity against the tested bacteria subsequent to the simulated digestion procedure, so digestion appears to have no decisive influence on the antibacterial properties of propolis. Some specific bacterial strains did not prove to be susceptible to propolis in certain concentrations.
Depending on the propolis concentration, the tested bacterial strains proved to be sensitive against the propolis samples of different geographical origin, except for E. coli. The largest inhibition zones were noticed for propolis denoted as “Z” and “B”, followed by “D” and “E” samples. Additionally the Gram negative Bacteroides fragilis showed susceptibility against bee glue. The soluble part of digested propolis samples did not inhibit the growth of E. coli strain, but at the same time it showed activity against all the other tested bacteria. Enterococcus faecium and E. coli displayed resistance against the insoluble part of the digested propolis, whereas the other investigated 10 bacteria exhibited sensitivity.
Based on our results it might be stated that the actual biological impact of propolis samples of diverse origin can just be accurately estimated if well-tailored model studies are performed on representative human intestinal bacteria. On the basis of the current knowledge, in this paper we pointed out the prospects of applicability of selected propolis samples for manufacturing functional foodstuffs of beneficial physiological features in the future.
Shri Krishna Govt. Ayurved College, Haryana
Time : 10:55-11:35
Dr. Shriniwas Gujjarwar is working as Professor and Head of Dept. of Shalyatantra (Surgery) under department of AYUSH, Govt. of Haryana at shri Krishna Govt. Ayurved College, Kurukshetra, Haryana. He is having 20 years of Academic and Clinical experience in the field of Ayurveda. He has completed his graduation and post graduation in Ayurveda from Dr. Babasaheb Ambedkar University, Aurangabad, Maharastra state with specialization in Shalyatantra. He is having vast knowledge and experience in academic and clinical field and is working as incharge to various academic committees and as a member to various committees constituted by the Govt.of Haryana and CCIM, AYUSH Ministry Govt. Of India. He has presented lectures on various topics as Guest Speaker/ Resource person at national, International conferences, Seminars, ROTPs and CMEs. He has been instrumental in organising seminars / Public conferences on different topics as an organising committee member.
Charaka, Sushruta and Vagbhata Samhita-texts are the fundamental trinity for explanation of deep knowledge regarding various aspects of preventing diseases and illustration of various treatments for various disease conditions in 5000BC. Ayurveda explains Ashtanga Ayurveda- eight pillars of different specialities of treatment for different types of ailments. These are kaya chikitsa- medicinal treatment, Bala chikitsa - paediatrics, Graha chikitsa - Psycho- somatic diseases, Urdhvaanga chikitsa -(Shalakya)- diseases pertaining to ear, nose, throat, mouth, eye, Shalya chikitsa - surgical treatments / surgeries, Danshtra- toxicology, Jara chikitsa - geriatrics and Vrisha chikitsa - science of aphrodisiacs. Basic texts of Ayurveda elucidate various types of treatments such as Shamana- medicinal treatment, shodhana-Panchakarma-body cleansing treatments along with some specialised treatments such as Kshara sutra treatment, Agnikarma- Heat therapy- cauterization, Jaluka treatment- leech therapy, Vrana chikitsa- wound management and shalya chikitsa – surgeries and fracture management. In this paper various examples of therapies of Ayurveda like Rejuvination, Panchakarma, Pottalli pindasweda, Agnikarma, Ksharasutra, Ksharkarma, jaloukavacharana, viddha Chikitsa etc. will be presented with their role in prevention and managment of various diseases. Also as probiotic effect of this therapis as well as Seasonal regimen (Ritucharya).