Day 3 :
Keynote Forum
Ascension Marcos
President of ISIN
Keynote: Effects of the consumption of Lactobacillus coryniformis CECT5711 strain on the immune response and intestinal function of healthy adults
Time : 10:00-10:30
Biography:
Ascension Marcos got her PhD at the School of Pharmacy at the Complutense University in Madrid, Spain (UCM) in 1982 and Master in Clinical Analysis by UCM in 1986, and got a grant at the Spanish National Research Council (CSIC). She was the Head of the Institute of Nutrition and Food Technology at the Mixed Center CSIC-UCM (1998-2002). She is the leader of the Immunonutrition Research Group at the Department of Metabolism and Nutrition at CSIC since 1987. She achieved the highest category at CSIC as a Research Professor in 2006 and her scientific consolidation has been recognized for 5 six-year terms since 1985. Shernis a pioneer in the field of Immunonutrition in Spain, Founder and President of the International Forum of Immunonutrition for Education and Research (i-FINER) since 2007. In 2014 the i-FINER group has developed the International Society for Immunonutrition (ISIN), she being also the President.
Abstract:
Background: Among the various health-promoting functions of probiotics, much attention has been paid to their immunological function, specifically the prevention of infections, which seems to be related with their capability to modulate de intestinal microbiota.
Objective: Since effects of probiotics are strain-dependent, the main objective of this study was to assess the effect of Lactobacillus coryniformis CECT5711 (Lc) on both immune response and intestinal function of healthy adults during 6 weeks of treatment in a Hepatitis A vaccine model.
Methods: One hundred twenty three volunteers were randomized into 1) Placebo group (n=40), which received a daily capsule containing maltodextrin, 2) Probiotic group (n=41), which received a daily capsule of Lc (109 cfu/day) and 3) Mixed group (n=42), which received Lc for 2wk (prior to vaccination) and then placebo. Blood and stool samples were taken in 3 visits (V): at the beginning (V1), after two weeks of treatment just before the vaccination (V2) and at the end of the study (V3). At each V, lymphocyte subset counts, phagocytic capacity, natural killer activity, serum cytokine levels and microbiota composition by qPCR were analyzed, whereas specific HAV antibodies were measured just before and after the vaccine by ELISA tests (at the beginning and at the end of the study). Intestinal habits (bowel movements and stool consistency with the Bristol scale: types 1-2 hard, types 3, 4 and 5 normal, types 6-7 soft watery) were evaluated by a dairy record, and the intestinal symptoms appearance (nausea, borborigms, bloating, intestinal pain and flatulence) weekly in a single specific day. One-way ANOVA with Bonferroni post-hoc tests and lineal mixed models were performed for normally-distributed variables and Kruskal-Wallis with Mann-Whitney U test, Friedman’s 2-way ANOVA and Kendall’s coefficient of concordance for non-parametric variables. Chi square test was used to evaluate the frequency of intestinal symptoms between groups of treatments and visits.
Results: Specific HAV antibodies were significantly higher in the mixed group compared to the control group (P=0.017). Memory T-helper lymphocytes increased in V3 vs V1 (P=0.032) in the probiotic group. No differences were found in innate immunity parameters and cytokine concentrations, as well as in the intestinal microbiota composition, bowel movements and stool consistency. However, a trend to a lower appearance of total symptoms were observed in the last 4 weeks of intervention in the probiotic and mixed groups compared to the placebo group (82.9% y 80.9 % vs 97.5%) (P=0.054).
Conclusion: These results suggest that Lactobacillus coryniformis CECT5711 could improve vaccine efficiency by enhancing increasing antibody response and perhaps immunological memory. There were no remarkable effects on the intestinal function probably because they are healthy adults with a very low appearance of intestinal disorder symptoms. Even so, the intervention with this strain led to a lower tendency of symptoms appearance compared to the placebo group.
Keynote Forum
Shugui Wang
Danone Nutricia Research, Singapore
Keynote: Will probiotics benefit brain development during the first 1000 days?
Time : 10:30-11:00
Biography:
Shugui Wang has completed her PhD at National University of Singapore. She worked as a Post-doctoral Researcher at Genome Institute of Singapore working closely with Martin Hibberd. Her research focus was on gut micro-biota in relation to host’s health and infection. Thereafter at the National Cancer Centre Singapore, she continued her research passion with Sven Pettersson on gut-brain axis focusing on gut micro-biota and brain functions. She is currently working at Danone Nutricia Research.
Abstract:
The acquisition of intestinal micro-biota after birth has a defining impact on the development and function of immune and metabolic systems integral to health and homeostasis. During first 1000 days of life, the gut micro-biota of infants quickly becomes remarkably diverse and rich. Interestingly, the neurodevelopmental window is paralleled with the major shifts in the infant micro-biota. Disruptions in evolving gut micro-biota during this critical period can impact postnatal brain development. Probiotics are believed to balance gut micro-biota and benefit the host’s health. They have been found to relieve mental disorders, possibly through lowering levels of pro-inflammatory cytokines in the host and decreasing stress-induced corticosterone production, whilst concurrently increasing bacterial metabolites such as tryptophan. It has been recently suggested that probiotic supplementation during the first six months of life may reduce the risk of development of neuropsychiatric disorders in later life. There is increasing evidence that probiotics contribute to brain development and especially during the first 1000 days of life. However, the mechanisms how probiotics benefit brain development still need further investigation.
Keynote Forum
Praveen Rishi
Panjab University
India
Keynote: Lactobacillus plantarum and EGCG in a synbox: an effective intervention for alcohol induced endotoxin mediated liver disease
Time : 10:55-11:20
Biography:
Praveen Rishi has completed her PhD from Post-graduate Institute of Medical Education and Research (PGIMER), India. Presently, she is the Chairperson of the Department of Microbiology, Panjab University, India. She has more than 100 publications in journals of repute and a patent to her credit. She is a Fellow of Association of Microbiologists of India (FAMI) and Fellow of Indian Association of Biomedical Scientists (FABMS). She is also a recipient of Dr. Y.S. Narayana Rao Oration Award, conferred by Indian Council of Medical Research (ICMR). She is serving as an Editorial Board Member of various journals of repute.
Abstract:
Alcohol abuse can cause substantial liver insufficiencies leading to significant mortality worldwide, regardless of the available therapeutic options. Recently, we have evaluated the use of alternative agents like Epigallocatechin gallate (EGCG) and L. plantarum to control ALD. The prebiotic property of EGCG for enhancing the growth of L. plantarum was evaluated. Based on this property a coupled formulation consisting of both EGCG and L. plantarum was developed by encapsulating these agents in Ca-alginate beads to achieve enhanced biological effects. The formulation was extensively characterized and evaluated for its enhanced in vivo efficiency. Effect of EGCG to enhance the growth of L. plantarum was significantly better than inulin. Combination beads lowered serum transaminases and blood alcohol levels. Alcohol fed rats elicited raised plasma endotoxin levels, attributable to the disrupted intestinal permeability, confirmed by lactulose-mannitol test using HPTLC. Transcription studies for TLR-4 receptor mediated signaling molecules (MyD88, CD14, MD2) revealed modulation in the expression of these molecules resulting in the down-regulation of NF-κB in rats treated with combination beads. Expression of pro-inflammatory cytokines i.e., TNF-α, and IL-12β/40 subunit, COX-2 in addition to the levels of antioxidants, oxidants and micronuclei formation also assumed normal levels. Histo-architecture depicted normal liver and intestine in rats treated with co-encapsulated beads whereas severely distorted histology was observed for respective tissues in alcoholic rats. Thus, the formulation of L. plantarum with EGCG in a synbox can be a promising therapeutic option ensuring enhanced bio-performance against ALD. To the best of our knowledge, these findings are being reported for the first time.
- Microbial Fermentation
- Introduction to Probiotics
- Microbes as Probiotics
- Types of Probiotics
Session Introduction
Farhan Saeed
GC University Faisalabad
Pakistan
Title: Effect of edible coating techniques on quality attributes of desi & farmi eggs
Time : 11:00-11:25
Biography:
Farhan Saeed has completed his Doctorate in 2012 in Food Technology discipline and has been working as Assistant Professor in Institute of Home and Food Sciences, Government College University Faisalabad since February, 2012. He has published 40 research and review articles in international well reputed journals having impact factor more than 80.
Abstract:
The core objective of current research work is to compare the nutritional profile of desi and farmi eggs and also observe the effects of different edible coatings on eggs for extending their shelf life. For the purpose, desi and farmi were procured from different farms. In 1st phase, nutritional profile i.e., protein content, fat contact, ash content, moisture, mineral determination of both types of eggs were determined through their respective methods. Moreover, in 2nd phase, two edible coatings i.e., shellac and chitosan were applied on egg shells. After coating, eggs were stored on normal conditions (room temperature) for four weeks and again evaluated for nutritional profile. Coatings effectively extended the shelf life of eggs when stored at room temperature by preserving its quality characteristics. Chitosan and shellac coatings minimized the weight loss and preserved the albumin and yolk quality as well as egg shell color of eggs for at least 3 week longer than those observed for non-coated eggs. Conclusively, coating materials have potential use in delaying charges in the interior quality of eggs and maintaining their shelf life.
Hassan Al-Demerdash
Suez Canal University
Egypt
Title: Detection of Bacterial Pathogens in Some Milk Products and Baby Foods Using A Specific PCR Assay
Time : 11:45-12:10
Biography:
Hassan A.M. El.Demerdash has completed his Ph.D. at the age of 29 years from Kiel University, Germany and postdoctoral studies from BAfM institute, Kiel, Germany. He has published more than 35 papers in reputed journals. He is the Head of Department of Food and Dairy Sciences and Technology, College of Agricultural and Environmental Sciences SCU, ARE. The main lines of her research are food biotechnology. His research activity is in the field of food supplementation; food fermentation; genetically modified foods; food waste management.
Abstract:
The present investigation studied the effects of some date palm (date-paste) and olive oil on the viability of probiotic starter culture (Lactobacillus acidophilus and Bifidobacterium breve) in fermented milk during manufacture and storage period. Date-paste and olive oil were added to fermented milk at different concentrations (based on a previous sensory analysis) as follows; 1, 1.5 and 2% for date-paste and 0.5, 1.5, 2.5% for olive oil respectively. Changes in chemical and viable total bacterial count of fermented milk were monitored during manufacture and storage at 4° C for 30 days. Data showed that the incubation time that was needed to reach pH 4.5 was considerably affected by the added ingredients. Also, the drop in pH or the increase in acidity of this fermented milk was dependent on the added ingredients and the percentage of supplementation. Results indicated that addition of date-paste and olive oil improved the viability of probiotic starter culture and the microbiological properties in the treated samples in comparison with control; the survival of probiotic bacteria in all treatments which were in viable counts remained at >107 cfu/g after 30 days of storage at 4° C. Moreover, addition of date palm-paste improved the viablity and stabilty of probiotic starter culutre than olive oil especially at the end of storage and the total viable counts were 7.1, 7.4, 7.7 and 7.5, 8.0 and 8.1×10-7 for olive oil and dates-paste at the end of storge, respectively. No clear difference was observed between the treatments in the effect on the fermented milk characteristics compared to the control one. Supplementation of the fermented milk with 2% date-paste and 1.5% olive oil improved the rheological properties and reduced the curd syneresis rate. Sensory evaluation showed that fermented milk fortified with 2% date-paste and 1.5% olive oil recorded the highest score and overall acceptability than the other treatments. It is suggested that the fermented milk of acceptable quality and high total probiotic bacterial count during storage can be made from milk supplemented with adjusted concentration (wt/vol) from date-paste and olive oil.
Shereen Lotfy Nassef
Agriculture Research Center
Egypt
Title: Functional and novel foods
Time : 12:10-12:35
Biography:
Dr. Shereen is currently Researcher at Agriculture Research Center, Department of Food Science And Technology, Egypt .She also lecturer at AUC-American University In Cairo . She was Associate Professor at, KSA King Faisal University K.S.A. Dr. Shereen earned her Ph.D. at the University of Zagazig, Egypt in the field of Food Science and Technology Department (2003 ) and did her Master at Cairo University Department of Home economics and Nutrition (1999) . She has published 10 peer reviewed research papers and one Book in Nutrition and health . Specific experience in the Nutrition work field, Attending & participation in conferences & workshops related to food science & nutrition, food and bakery free gluten & food safety. Research work in the field of food safety, food and bakery and nutrition. Ongoing project of relation of food safety awareness, food pollution & functional food (Functional bakery products). Experienced teaching activities , Postgraduate & undergraduate subjects related to nutrition food safety and general health, Lecturing in the courses held by The King Faisal University & Agriculture Research Center in topics related to Nutrition , bakery products, free gluten & food safety.
Abstract:
Functional foods began in Japan in 1980, in year 1991 which published that some foods have health benefits and it has been proved this claim. In America in 1993 it has been proven with results that it help in reducing the risk of diseases also the FDA agreed on the relationship between components of food and pathogens. The World Council food information which has cleared and defined functional food as food ingredients that improve physiological function and health in addition to their nutritional value. Novel food and is known as a type of food that does not have a significant history of consumption or a result of the method never before used in food. Some reasons for the spread of functional foods are to increase awareness of food and health, an increase in life expectancy for the elderly, the difference between each of prebiotics and probiotics, show some examples for functional and novel food and important definitions, explain the relationship of the active ingredients in food and color of food and food sources and the safety of functional and novel foods. From these, it is clear that "Functional and Novel Foods" is a sensitive issue and influential sectors of health, industry, directly and indirectly and on the other sectors, multi economically and socially as it represents a good area of scientific creativity should attract serious scholars and researchers in the field of scientific research.
Luis Gosalbez
Biopolis S.L.
Spain
Title: Key regulatory issues in the development of pharmabiotics in Europe
Time : 12:35-13:00
Biography:
Luis is the Manager of Strategic Projects at Biopolis S.L. in Valencia, Spain. Previously, he was a Research Associate in Universidad Complutense de Madrid and in Centro de Biología Molecular Severo Ochoa-CSIC (both in Spain) and in the Korea Advanced Institute of Science and Technology (KAIST, South Korea). His research was always focused on microbiology, development of antimicrobials and antivirals. He also served as Senior Analyst for a leading Life Sciences and MedTech strategy and operations consulting firm in Munich (Germany). He received his BSc and MSc in Biotechnology from Universidad Complutense de Madrid and a Master’s in Bioscience Enterprise from the University of Cambridge in the UK.
Abstract:
Microorganisms as drugs are not regulated consistently among EU Member States, which makes investment in R&D too risky for companies. Both the industry and regulators are interested in working on legislation together in order to enable developments in this area. Pharmabiotics/Live Biotherapeutic Products are a novel regulatory category in the European pharmaceutical landscape. Challenges in terms of the scope of the regulation, safety and efficacy standards are amongst the most important to be dealt with in these early discussions.